Wavelength: 280nm
Mobile Phase: Methanol/(glacial acetic acid /0.14% NaAC solution =1/40)=70/30
Temp.: 30°C
Flow Rate: 1.0mL/min
CN HPLC Columns
Description
CN HPLC Columns Description
CN HPLC columns, which use Hawach Scientific’s unique innovative filler bonding process, are based on ultra-high purity fully porous spherical silica gel, bonded with cyanopropyl functional groups, and controlled by the strict process to ensure the formation of uniform, dense amino-functional molecules. Layers maximize bond coverage, and ensure that the column packing has excellent stability.
The reversed-phase cyano HPLC column elutes rapidly for hydrophobic molecules, has unique selectivity for polar compounds, and has a good peak shape for the separation of strong basic compounds (including ammonium salts). It has different selectivity from reversed-phase columns such as C18 and C8. A cyano column can be used when a highly hydrophobic compound cannot be eluted with a standard C18 and C8 column or a typical reversed-phase eluent.
The normal phase column can replace the silica gel column, which has a fast equilibrium and is more consistent than the activity of the non-derivatized silica gel. It can replace most of the cyano liquid chromatography columns on the market.
Cyano HPLC Columns Characteristics
Can be applied in both positive and negative phases;
Stable high coverage bonded phase;
Lower hydrophobicity with unique selectivity
Parameters
Bonded phase: cyanopropyl
Particle size: 3μm, 5μm, 10μm
Aperture: 120Å
Specific surface area: 320m²/g
Sealing: Sealing
pH stability: 1.5 to 9.0
Technical Data
| Bonded Phase | USP No | Particle size (μm) | Pore size | Surface Area (m2/g) | Carbon Loading | pH Range | End-capped |
| CN | L10 | 5 | 120Å | 320 | 6% | 1.5-10 | No |
Ordering Information
| Item No | Description | Package (pcs/pk) |
|---|---|---|
| SLHCN32130 | HPLC Column, CN, 3μm, 120Å, 2.1x30mm | 1 |
| SLHCN32133 | HPLC Column, CN, 3μm, 120Å, 2.1x33mm | 1 |
| SLHCN32150 | HPLC Column, CN, 3μm, 120Å, 2.1x50mm | 1 |
| SLHCN32175 | HPLC Column, CN, 3μm, 120Å, 2.1x75mm | 1 |
| SLHCN321100 | HPLC Column, CN, 3μm, 120Å, 2.1x100mm | 1 |
| SLHCN321125 | HPLC Column, CN, 3μm, 120Å, 2.1x125mm | 1 |
| SLHCN321150 | HPLC Column, CN, 3μm, 120Å, 2.1x150mm | 1 |
| SLHCN321200 | HPLC Column, CN, 3μm, 120Å, 2.1x200mm | 1 |
| SLHCN321250 | HPLC Column, CN, 3μm, 120Å, 2.1x250mm | 1 |
| SLHCN33030 | HPLC Column, CN, 3μm, 120Å, 3.0x30mm | 1 |
| SLHCN33050 | HPLC Column, CN, 3μm, 120Å, 3.0x50mm | 1 |
| SLHCN33075 | HPLC Column, CN, 3μm, 120Å, 3.0x75mm | 1 |
| SLHCN330100 | HPLC Column, CN, 3μm, 120Å, 3.0x100mm | 1 |
| SLHCN330125 | HPLC Column, CN, 3μm, 120Å, 3.0x125mm | 1 |
| SLHCN330150 | HPLC Column, CN, 3μm, 120Å, 3.0x150mm | 1 |
| SLHCN330200 | HPLC Column, CN, 3μm, 120Å, 3.0x200mm | 1 |
| SLHCN330250 | HPLC Column, CN, 3μm, 120Å, 3.0x250mm | 1 |
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