HILIC Amide HPLC Columns

Detector: RID (40°C)
Temperature: 40°C
Flow Rate: 1.0mL/min
Injection Volume: 20μl


HILIC Amide HPLC Columns Description

The HILIC column adopts a polar modified stationary phase, which enhances the retention capacity of some strongly polar compounds and effectively overcomes the shortcomings of reversed-phase chromatography of poor retention of such compounds. Unlike traditional reversed-phase chromatography columns, HILIC columns only require a small amount of water in the mobile phase to retain strongly polar compounds, and the increase in the organic phase is beneficial to improve the detection sensitivity of compounds

HILIC amide HPLC column, the stationary phase is based on ultra-pure fully porous spherical silica gel, using the unique phase-bonding technology of Hawach Scientific, bonding carbamoyl functional groups, through special surface modification technology, very good. Its hydrophilicity and stability have a longer life than the amino column. It can effectively separate sugars, peptides, and low molecular weight polar compounds, and is the best choice for replacing the amino column in hilic mode.

HILIC Amide HPLC Columns Characteristics

Uniform pore size distribution and uniform hydrophilic polymer covering the surface of the silica gel enables efficient separation of highly polar and hydrophilic compounds;
It can be used as a column for hydrophobic interaction analysis and is widely used in the analysis of peptides, nucleotides, glycosides, and hydrophobic drugs;
A unique bonding process to ensure that the stationary phase is more stable in aqueous organic mobile phases;
Suitable for LC/MS analysis of water-soluble polar compounds.

HILIC Amide HPLC Columns Parameters

Bonded phase: carbamoyl
Aperture: 120Å
Carbon load: 7%
Particle size: 3μm, 5μm
Sealing: no closure
Specific surface area: 320m2/g
pH stability: 1.5 to 10.0

Technical Data

Bonded PhaseUSP NoParticle size (μm)Pore sizeSurface Area (m2/g)Carbon
pH RangeEnd-capped
HILIC Amide/5μm120320 7%2.0-8.0No

Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHHA546100HPLC Column, HILIC Amide, 5μm, 120Å, 4.6x100mm1
SLHHA546150HPLC Column, HILIC Amide, 5μm, 120Å, 4.6x150mm1
SLHHA546250HPLC Column, HILIC Amide, 5μm, 120Å, 4.6x250mm1
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