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Reversed Phase C4 HPLC Columns

Detection Wavelength: 215nm
Mobile Phase:
A: 0.1% TFA water solution
B: 0.1% trifluoroacetic acid acetonitrile solution
Temperature: 30°C

Description

Description of C4 HPLC Columns

Reversed Phase C4 HPLC Columns have strong retention ability for hydrophobic and polar compounds, unique bonding technology, high bonding phase coverage, unique double tail sealing technology, which minimizes residual silicon hydroxy influence, alkaline and strong The separation of polar compounds have a good peak shape and can provide a pore size of 300 Å. It is applied to the separation and analysis of biological proteins, and the peak shape is sharp and super high.

The surface hydrophobicity of the stationary phase of the C4 HPLC column is lower than that of the ODS HPLC column and C8 HPLC column, so the sample retention time is also shorter than that of ODS and C8.

Reversed Phase C4 HPLC Columns Technical Parameters

Reverse phase column
Particle size: 3μm, 5μm
Aperture: 120Å, 300Å
Specific surface area: 120Å–320m²/g, 300Å– 90m²/g
Carbon loading: 120Å-8%, 300Å–3%
pH range: 1.5-10.0
Whether to seal: yes

Reversed Phase C4 HPLC Columns Precautions

1. The pH of the Polar-RP HPLC column ranges from 1.5 to 10.0. It is generally recommended to use a pH range of 2.0 to 8.0. If the mobile phase is outside the pH range, the silica matrix will be lost and the bonded phase will be broken. Shorten the service life, should strictly control the range of pH value;
2. The highest operating temperature is 60℃, and the recommended operating temperature is lower than 40°C.

Technical Data

Bonded Phase USP No Particle size (μm) Pore size Surface Area (m²/g) Carbon
Loading
pH Range End-capped
 C4 L26 3 μm, 5μm 120 320 8% 1.5-10 Yes

Ordering Information

Item No Description Package (pcs/pk)
SLHC432130 HPLC Column, C4, 3μm, 120Å, 2.1x30mm 1
SLHC432133 HPLC Column, C4, 3μm, 120Å, 2.1x33mm 1
SLHC432150 HPLC Column, C4, 3μm, 120Å, 2.1x50mm 1
SLHC432175 HPLC Column, C4, 3μm, 120Å, 2.1x75mm 1
SLHC4321100 HPLC Column, C4, 3μm, 120Å, 2.1x100mm 1
SLHC4321125 HPLC Column, C4, 3μm, 120Å, 2.1x125mm 1
SLHC4321150 HPLC Column, C4, 3μm, 120Å, 2.1x150mm 1
SLHC4321200 HPLC Column, C4, 3μm, 120Å, 2.1x200mm 1
SLHC4321250 HPLC Column, C4, 3μm, 120Å, 2.1x250mm 1
SLHC433030 HPLC Column, C4, 3μm, 120Å, 3.0x30mm 1
SLHC433050 HPLC Column, C4, 3μm, 120Å, 3.0x50mm 1
SLHC433075 HPLC Column, C4, 3μm, 120Å, 3.0x75mm 1
SLHC4330100 HPLC Column, C4, 3μm, 120Å, 3.0x100mm 1
SLHC4330125 HPLC Column, C4, 3μm, 120Å, 3.0x125mm 1
SLHC4330150 HPLC Column, C4, 3μm, 120Å, 3.0x150mm 1
SLHC4330200 HPLC Column, C4, 3μm, 120Å, 3.0x200mm 1
SLHC4330250 HPLC Column, C4, 3μm, 120Å, 3.0x250mm 1
SLHC434030 HPLC Column, C4, 3μm, 120Å, 4.0x30mm 1
SLHC434050 HPLC Column, C4, 3μm, 120Å, 4.0x50mm 1
SLHC434075 HPLC Column, C4, 3μm, 120Å, 4.0x75mm 1
SLHC4340100 HPLC Column, C4, 3μm, 120Å, 4.0x100mm 1
SLHC4340125 HPLC Column, C4, 3μm, 120Å, 4.0x125mm 1
SLHC4339150 HPLC Column, C4, 3μm, 120Å, 3.9x150mm 1
SLHC4340150 HPLC Column, C4, 3μm, 120Å, 4.0x150mm 1
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