Reversed Phase C4 HPLC Column

Detection Wavelength: 215nm
Mobile Phase:
A: 0.1% TFA water solution
B: 0.1% trifluoroacetic acid acetonitrile solution
Temperature: 30°C

Availability: In Stock


Description of C4 Column

Hawach reversed phase C4 column, with its unique bonding technology, high bonded phase coverage, and unique double-end sealing technology, minimizes the impact of residual silanol.

It has a strong retention ability for hydrophobic and polar compounds, strong basicity, and good peak shape for the separation of polar compounds.

Pore sizes up to 300 Å are available. Applied to the separation and analysis of biological proteins, the peak shape is sharp and ultra-high.

Technical Parameters

  • Reverse phase column
  • Particle size: 3μm, 5μm
  • Aperture: 120Å, 300Å
  • Specific surface area: 120Å–320m²/g, 300Å– 90m²/g
  • Carbon loading: 120Å-8%, 300Å–3%
  • pH range: 1.5-10.0
  • Whether to seal: yes

Precautions of Reversed Phase C4 Column

  1. The pH of the Polar-RP HPLC column ranges from 1.5 to 10.0. It is generally recommended to use a pH range of 2.0 to 8.0. If the mobile phase is outside the pH range, the silica matrix will be lost and the bonded phase will be broken. Shorten the service life, should strictly control the range of pH value;
  2. The highest operating temperature is 60℃, and the recommended operating temperature is lower than 40°C.

The surface hydrophobicity of the stationary phase of the C4 HPLC column is lower than that of the ODS HPLC column and C8 HPLC column, so the sample retention time is also shorter than that of ODS and C8.

Technical Data

Bonded PhaseUSP NoParticle size (μm)Pore sizeSurface Area (m²/g)Carbon
pH RangeEnd-capped
 C4L263 μm, 5μm1203208%1.5-10Yes

Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHC432130C4, 3μm, 120Å, 2.1*30mm1
SLHC432133C4, 3μm, 120Å, 2.1*33mm1
SLHC432150C4, 3μm, 120Å, 2.1*50mm1
SLHC432175C4, 3μm, 120Å, 2.1*75mm1
SLHC4321100C4, 3μm, 120Å, 2.1*100mm1
SLHC4321125C4, 3μm, 120Å, 2.1*125mm1
SLHC4321150C4, 3μm, 120Å, 2.1*150mm1
SLHC4321200C4, 3μm, 120Å, 2.1*200mm1
SLHC4321250C4, 3μm, 120Å, 2.1*250mm1
SLHC433030C4, 3μm, 120Å, 3.0*30mm1
SLHC433050C4, 3μm, 120Å, 3.0*50mm1
SLHC433075C4, 3μm, 120Å, 3.0*75mm1
SLHC4330100C4, 3μm, 120Å, 3.0*100mm1
SLHC4330125C4, 3μm, 120Å, 3.0*125mm1
SLHC4330150C4, 3μm, 120Å, 3.0*150mm1
SLHC4330200C4, 3μm, 120Å, 3.0*200mm1
SLHC4330250C4, 3μm, 120Å, 3.0*250mm1
SLHC434030C4, 3μm, 120Å, 4.0*30mm1
SLHC434050C4, 3μm, 120Å, 4.0*50mm1
SLHC434075C4, 3μm, 120Å, 4.0*75mm1
SLHC4340100C4, 3μm, 120Å, 4.0*100mm1
SLHC4340125C4, 3μm, 120Å, 4.0*125mm1
SLHC4339150C4, 3μm, 120Å, 3.9*150mm1
SLHC4340150C4, 3μm, 120Å, 4.0*150mm1
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