Detection Wavelength: 215nm
Mobile Phase:
A: 0.1% TFA water solution
B: 0.1% trifluoroacetic acid acetonitrile solution
Temperature: 30°C
Reversed Phase C4 HPLC Columns
Description
Description of C4 HPLC Columns
Reversed Phase C4 HPLC Columns have strong retention ability for hydrophobic and polar compounds, unique bonding technology, high bonding phase coverage, unique double tail sealing technology, which minimizes residual silicon hydroxy influence, alkaline and strong The separation of polar compounds have a good peak shape and can provide a pore size of 300 Å. It is applied to the separation and analysis of biological proteins, and the peak shape is sharp and super high.
The surface hydrophobicity of the stationary phase of the C4 HPLC column is lower than that of the ODS HPLC column and C8 HPLC column, so the sample retention time is also shorter than that of ODS and C8.
Reversed Phase C4 HPLC Columns Technical Parameters
Reverse phase column
Particle size: 3μm, 5μm
Aperture: 120Å, 300Å
Specific surface area: 120Å–320m²/g, 300Å– 90m²/g
Carbon loading: 120Å-8%, 300Å–3%
pH range: 1.5-10.0
Whether to seal: yes
Reversed Phase C4 HPLC Columns Precautions
1. The pH of the Polar-RP HPLC column ranges from 1.5 to 10.0. It is generally recommended to use a pH range of 2.0 to 8.0. If the mobile phase is outside the pH range, the silica matrix will be lost and the bonded phase will be broken. Shorten the service life, should strictly control the range of pH value;
2. The highest operating temperature is 60℃, and the recommended operating temperature is lower than 40°C.
Technical Data
| Bonded Phase | USP No | Particle size (μm) | Pore size | Surface Area (m²/g) | Carbon Loading | pH Range | End-capped |
| C4 | L26 | 3 μm, 5μm | 120 | 320 | 8% | 1.5-10 | Yes |
Ordering Information
| Item No | Description | Package (pcs/pk) |
|---|---|---|
| SLHC432130 | HPLC Column, C4, 3μm, 120Å, 2.1x30mm | 1 |
| SLHC432133 | HPLC Column, C4, 3μm, 120Å, 2.1x33mm | 1 |
| SLHC432150 | HPLC Column, C4, 3μm, 120Å, 2.1x50mm | 1 |
| SLHC432175 | HPLC Column, C4, 3μm, 120Å, 2.1x75mm | 1 |
| SLHC4321100 | HPLC Column, C4, 3μm, 120Å, 2.1x100mm | 1 |
| SLHC4321125 | HPLC Column, C4, 3μm, 120Å, 2.1x125mm | 1 |
| SLHC4321150 | HPLC Column, C4, 3μm, 120Å, 2.1x150mm | 1 |
| SLHC4321200 | HPLC Column, C4, 3μm, 120Å, 2.1x200mm | 1 |
| SLHC4321250 | HPLC Column, C4, 3μm, 120Å, 2.1x250mm | 1 |
| SLHC433030 | HPLC Column, C4, 3μm, 120Å, 3.0x30mm | 1 |
| SLHC433050 | HPLC Column, C4, 3μm, 120Å, 3.0x50mm | 1 |
| SLHC433075 | HPLC Column, C4, 3μm, 120Å, 3.0x75mm | 1 |
| SLHC4330100 | HPLC Column, C4, 3μm, 120Å, 3.0x100mm | 1 |
| SLHC4330125 | HPLC Column, C4, 3μm, 120Å, 3.0x125mm | 1 |
| SLHC4330150 | HPLC Column, C4, 3μm, 120Å, 3.0x150mm | 1 |
| SLHC4330200 | HPLC Column, C4, 3μm, 120Å, 3.0x200mm | 1 |
| SLHC4330250 | HPLC Column, C4, 3μm, 120Å, 3.0x250mm | 1 |
| SLHC434030 | HPLC Column, C4, 3μm, 120Å, 4.0x30mm | 1 |
| SLHC434050 | HPLC Column, C4, 3μm, 120Å, 4.0x50mm | 1 |
| SLHC434075 | HPLC Column, C4, 3μm, 120Å, 4.0x75mm | 1 |
| SLHC4340100 | HPLC Column, C4, 3μm, 120Å, 4.0x100mm | 1 |
| SLHC4340125 | HPLC Column, C4, 3μm, 120Å, 4.0x125mm | 1 |
| SLHC4339150 | HPLC Column, C4, 3μm, 120Å, 3.9x150mm | 1 |
| SLHC4340150 | HPLC Column, C4, 3μm, 120Å, 4.0x150mm | 1 |
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