:Home/HPLC Columns/Reversed Phase C4 HPLC Columns

Reversed Phase C4 HPLC Columns

Detection Wavelength: 215nm
Mobile Phase:
A: 0.1% TFA water solution
B: 0.1% trifluoroacetic acid acetonitrile solution
Temperature: 30°C

Description

Description of C4 HPLC Columns

Reversed Phase C4 HPLC Columns have strong retention ability for hydrophobic and polar compounds, unique bonding technology, high bonding phase coverage, unique double tail sealing technology, which minimizes residual silicon hydroxy influence, alkaline and strong The separation of polar compounds have a good peak shape and can provide a pore size of 300 Å. It is applied to the separation and analysis of biological proteins, and the peak shape is sharp and super high.

The surface hydrophobicity of the stationary phase of the C4 HPLC column is lower than that of the ODS HPLC column and C8 HPLC column, so the sample retention time is also shorter than that of ODS and C8.

Reversed Phase C4 HPLC Columns Technical Parameters

Reverse phase column
Particle size: 3μm, 5μm
Aperture: 120Å, 300Å
Specific surface area: 120Å–320m²/g, 300Å– 90m²/g
Carbon loading: 120Å-8%, 300Å–3%
pH range: 1.5-10.0
Whether to seal: yes

Reversed Phase C4 HPLC Columns Precautions

1. The pH of the Polar-RP HPLC column ranges from 1.5 to 10.0. It is generally recommended to use a pH range of 2.0 to 8.0. If the mobile phase is outside the pH range, the silica matrix will be lost and the bonded phase will be broken. Shorten the service life, should strictly control the range of pH value;
2. The highest operating temperature is 60℃, and the recommended operating temperature is lower than 40°C.

Technical Data

Bonded PhaseUSP NoParticle size (μm)Pore sizeSurface Area (m²/g)Carbon
Loading
pH RangeEnd-capped
 C4L263 μm, 5μm1203208%1.5-10Yes

Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHC432130HPLC Column, C4, 3μm, 120Å, 2.1x30mm1
SLHC432133HPLC Column, C4, 3μm, 120Å, 2.1x33mm1
SLHC432150HPLC Column, C4, 3μm, 120Å, 2.1x50mm1
SLHC432175HPLC Column, C4, 3μm, 120Å, 2.1x75mm1
SLHC4321100HPLC Column, C4, 3μm, 120Å, 2.1x100mm1
SLHC4321125HPLC Column, C4, 3μm, 120Å, 2.1x125mm1
SLHC4321150HPLC Column, C4, 3μm, 120Å, 2.1x150mm1
SLHC4321200HPLC Column, C4, 3μm, 120Å, 2.1x200mm1
SLHC4321250HPLC Column, C4, 3μm, 120Å, 2.1x250mm1
SLHC433030HPLC Column, C4, 3μm, 120Å, 3.0x30mm1
SLHC433050HPLC Column, C4, 3μm, 120Å, 3.0x50mm1
SLHC433075HPLC Column, C4, 3μm, 120Å, 3.0x75mm1
SLHC4330100HPLC Column, C4, 3μm, 120Å, 3.0x100mm1
SLHC4330125HPLC Column, C4, 3μm, 120Å, 3.0x125mm1
SLHC4330150HPLC Column, C4, 3μm, 120Å, 3.0x150mm1
SLHC4330200HPLC Column, C4, 3μm, 120Å, 3.0x200mm1
SLHC4330250HPLC Column, C4, 3μm, 120Å, 3.0x250mm1
SLHC434030HPLC Column, C4, 3μm, 120Å, 4.0x30mm1
SLHC434050HPLC Column, C4, 3μm, 120Å, 4.0x50mm1
SLHC434075HPLC Column, C4, 3μm, 120Å, 4.0x75mm1
SLHC4340100HPLC Column, C4, 3μm, 120Å, 4.0x100mm1
SLHC4340125HPLC Column, C4, 3μm, 120Å, 4.0x125mm1
SLHC4339150HPLC Column, C4, 3μm, 120Å, 3.9x150mm1
SLHC4340150HPLC Column, C4, 3μm, 120Å, 4.0x150mm1
CONTACT FORM
Enter your email address and our sales staff will contact you as soon as possible.


    Go to Top