Description of PFP HPLC Column
A PFP(pentafluorophenyl) HPLC column was obtained by substituting a fluorine atom for five hydrogen atoms on a phenyl column. In addition to high selectivity to fluorine-containing and halogen-containing compounds, the fluorine-containing stationary phase can also be used as a general reversed-phase stationary phase for the separation of halogen-free or chlorine-free halogen compounds to provide a CH alkyl group. The selectivity of the stationary phase is different. Widely used in RP column HPLC like biopharmaceuticals, hot products, and environmental analysis.
The fluorinated stationary phase, H on the benzene ring is substituted by F and has stronger ion exchange and polarity separation characteristics than in the alkyl stationary phase. Separation can be achieved: separation of phenols, aromatics and amines, steroids, and natural products of paclitaxel.
Application of PFP HPLC Column
1. Separation of natural products for fluorochemicals and paclitaxel
2. PFP also has high selectivity for aromatic compounds.
3. Can retain basic compounds, lower than the hydrophobicity of C18
4. Can be in reverse phase, HILIC
5. Can operate in 100% pure water mode
6. Stable, low loss, suitable for LC-MS, LC-UV
Characteristics of PFP HPLC Column
Good separation selectivity for halogen-containing substances and structural isomers;
Providing a different selectivity than the alkane stationary phase;
Very strong geometric size and stereoscopic shape selectivity;
A compound capable of separating a positional isomer structure or an alkyl stationary phase that is difficult to separate.
Bonded phase: PFP (USP L11/L43)
Particle size: 3μm, 5μm
Specific surface area: 320m2/g (120Å)
Carbon loading: C: 13% (120Å), F: 7% (120Å)
Sealing: Double tailing
pH stability: 1.5-10.0.