Description of PFP HPLC Column
A PFP(pentafluorophenyl) HPLC column was obtained by substituting a fluorine atom for five hydrogen atoms on a phenyl column. In addition to high selectivity to fluorine-containing and halogen-containing compounds, the fluorine-containing stationary phase can also be used as a general reversed-phase stationary phase for the separation of halogen-free or chlorine-free halogen compounds to provide a CH alkyl group. The selectivity of the stationary phase is different. Widely used in RP column HPLC like biopharmaceuticals, hot products, and environmental analysis.
The fluorinated stationary phase, H on the benzene ring is substituted by F and has stronger ion exchange and polarity separation characteristics than in the alkyl stationary phase. Separation can be achieved: separation of phenols, aromatics and amines, steroids, and natural products of paclitaxel.
Application of PFP HPLC Column
- Separation of natural products for fluorochemicals and paclitaxel
- PFP also has high selectivity for aromatic compounds.
- Can retain basic compounds, lower than the hydrophobicity of C18
- Can be in reverse phase, HILIC
- Can operate in 100% pure water mode
- Stable, low loss, suitable for LC-MS, LC-UV
Characteristics of PFP HPLC Column
Good separation selectivity for halogen-containing substances and structural isomers;
Providing a different selectivity than the alkane stationary phase;
Very strong geometric size and stereoscopic shape selectivity;
A compound capable of separating a positional isomer structure or an alkyl stationary phase that is difficult to separate.
Bonded phase: PFP (USP L11/L43)
Particle size: 3μm, 5μm
Specific surface area: 320m2/g (120Å)
Carbon loading: C: 13% (120Å), F: 7% (120Å)
Sealing: Double tailing
pH stability: 1.5-10.0.