SiO2 HPLC Columns

SiO2 HPLC Columns Description

The SiO2 (silica gel) HPLC column uses B-type ultra-pure fully porous spherical silica gel with low acidity and low metal content. SiO2 HPLC Columns can be used for the separation of strongly hydrophilic compounds in a reverse phase mode with a high organic phase, and is particularly suitable for the separation of polar compounds which produce tailing on other company SiO2 columns.
SiO2 liquid chromatography column with B type ultra-pure fully porous spherical silica gel with low acidic, low metal content characteristics.
SiO2 is suitable for most normal phase chromatography applications and as a stationary phase for Hilic Hydrophilic Interaction Chromatography. It is especially suitable for the separation of polar compounds that cause tailing on other companies’ silica gel columns, and can replace most of the silica gel liquid chromatography columns on the market.

SiO2 HPLC Columns Features

Type B ultra-pure, fully porous spherical silica gel with low acidity and low metal content
pH range: 2.0 to 8.0
Excellent batch and batch reproducibility

SiO2 HPLC Columns Technical Parameters

Particle size: 3μm, 5μm, 10μm
Aperture: 120Å
Specific surface area: 320m²/g
PH stability 2.0～8.0
Whether to seal: No

Routine maintenance

Positive phase use:
It is recommended to filter the sample and mobile phase before testing;
It is recommended to clean the samples in time after completing the samples;
After daily use, rinse the mobile phase with 10 to 20 column volumes or 2 times the retention time of the last peak in the experiment.

Column storage

1) Preservation of the column in a short time:
If the use interval is not more than four days, store in an organic solvent of the same composition that does not contain acid or alkali.
2) Preservation of long-term columns:
After rinsing 20 times column volume with isopropyl alcohol and the mobile phase (n-hexane/isopropanol = 99.5/0.5) indicated in the product quality report, remove it from the HPLC instrument and seal it with a plastic stopper. Store in a cool, dry place to avoid solvent evaporation from the column.

Inverted Use

It is recommended to filter the sample and mobile phase before testing;
It is recommended to clean the samples in time after completing the samples;
Conventional mobile phase (without buffer) conditions: can be directly switched to mobile phase detection before use. After the test is completed, rinse 10~20 times column volume with methanol or acetonitrile;
Use buffer conditions: Flush the column with 10 times the column volume using a transitional mobile phase before using the buffer.
Then switch to the mobile phase detection sample. After the detection is completed, it is also necessary to use the transition mobile phase to wash the column 20 times column volume, and then rinse the column with 10 to 20 times with methanol or acetonitrile.

Technical Data

Ordering Information