Wavelength: 220nm
Temp.: Ambient
Flow Rate: 1.0mL/min
Injection Volume: 5μL
C8-Universal HPLC Columns
Description
C8-Universal HPLC Columns Description
The C8-Universal HPLC column is based on ultra-pure silica gel for a thorough double-end bonding process. C8 column has classical reverse phase retention and selectivity that is slightly less than the C18 column retention capacity and is therefore recommended for separation on highly hydrophobic compounds and LC/MS instruments. C8 requires less analysis time than C18 when neutralizing neutral or other compounds with strong retention under the same chromatographic conditions.
1. A liquid phase analytical column with a slightly weaker retention capacity than C18
2. Wide pH tolerance range: 1.5-10
3. Peak shape, super high efficiency
C8-Universal HPLC Columns Parameters
Non polar HPLC column
Bonded phase: C8 (USP L7)
Particle size: 3μm, 5μm, 10μm
Aperture: 120Å
Specific surface area: 320m²/g (120Å)
Carbon loading: 12% (120Å)
Sealing: Double tailing
pH: value stability 1.5-10.0
New column activation
Rinsing with 80% methanol 0.5ml/min for 4 hours, then change the liquid to analyze the flow phase balance;
If the mobile phase contains buffer salts, please use the transitional mobile phase transition and then analyze the mobile phase balance.
Technical Data
| Bonded Phase | USP No | Particle size (μm) | Pore size | Surface Area (m²/g) | Carbon Loading |
PH Range | End-capped |
| C8-Universal | L7 | 1.8 µm,3 µm, 5µm,10 µm | 120Å, 300Å |
120Å –320; 300Å–90 |
12% | 1.5-10 | Yes |
Ordering Information
| Item No | Description | Package (pcs/pk) |
|---|---|---|
| SLHC8U32130 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x30mm | 1 |
| SLHC8U32133 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x33mm | 1 |
| SLHC8U32150 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x50mm | 1 |
| SLHC8U32175 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x75mm | 1 |
| SLHC8U321100 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x100mm | 1 |
| SLHC8U321125 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x125mm | 1 |
| SLHC8U321150 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x150mm | 1 |
| SLHC8U321200 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x200mm | 1 |
| SLHC8U321250 | HPLC Column, C8-Universal, 3μm, 120Å, 2.1x250mm | 1 |
| SLHC8U33030 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x30mm | 1 |
| SLHC8U33050 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x50mm | 1 |
| SLHC8U33075 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x75mm | 1 |
| SLHC8U330100 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x100mm | 1 |
| SLHC8U330125 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x125mm | 1 |
| SLHC8U330150 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x150mm | 1 |
| SLHC8U330200 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x200mm | 1 |
| SLHC8U330250 | HPLC Column, C8-Universal, 3μm, 120Å, 3.0x250mm | 1 |
| SLHC8U34040 | HPLC Column, C8-Universal, 3μm, 120Å, 4.0x30mm | 1 |
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