Wavelength: 220 nm
Temp.: Ambient
Flow Rate: 1.0mL/min
Injection Volume: 5μL
Availability: In Stock
C8-Universal HPLC Column
Description
Description of Universal C8 Column
The Hawach universal HPLC C8 column is based on ultra-pure silica gel for a thorough double-end bonding process.
The C8 column has classical reverse-phase retention and selectivity that is slightly less than the C18 column retention capacity and is therefore recommended for separation on highly hydrophobic compounds and LC/MS instruments. C8 requires less analysis time than C18 when neutralizing neutral or other compounds with strong retention under the same chromatographic conditions.
- A liquid phase analytical column with a slightly weaker retention capacity than C18;
- Wide pH tolerance range: 1.5-10;
- Peak shape, super high efficiency.
Universal C8 Column Parameters
- Non-polar HPLC column
- Bonded phase: C8 (USP L7)
- Particle size: 3μm, 5μm, 10μm
- Aperture: 120Å
- Specific surface area: 320m²/g (120Å)
- Carbon loading: 12% (120Å)
- Sealing: Double tailing
- pH: value stability 1.5-10.0
Applications
The C8 HPLC column is more suitable for analyzing macromolecular substances, such as some globulins, etc., which are used in conjunction with C8 and a buffer salt eluent.
New column activation
Rinsing with 80% methanol 0.5ml/min for 4 hours, then change the liquid to analyze the flow phase balance;
If the mobile phase contains buffer salts, please use the transitional mobile phase transition and then analyze the mobile phase balance.
Technical Data
| Bonded Phase | USP No | Particle size (μm) | Pore size | Surface Area (m²/g) | Carbon Loading | PH Range | End-capped |
| C8-Universal | L7 | 1.8 µm,3 µm, 5µm,10 µm | 120Å, 300Å | 120Å –320; 300Å–90 | 12% | 1.5-10 | Yes |
Ordering Information
| Item No | Description | Package (pcs/pk) |
|---|---|---|
| SLHC8U32130 | C8-Universal, 3μm, 120Å, 2.1x30mm | 1 |
| SLHC8U32133 | C8-Universal, 3μm, 120Å, 2.1x33mm | 1 |
| SLHC8U32150 | C8-Universal, 3μm, 120Å, 2.1x50mm | 1 |
| SLHC8U32175 | C8-Universal, 3μm, 120Å, 2.1x75mm | 1 |
| SLHC8U321100 | C8-Universal, 3μm, 120Å, 2.1x100mm | 1 |
| SLHC8U321125 | C8-Universal, 3μm, 120Å, 2.1x125mm | 1 |
| SLHC8U321150 | C8-Universal, 3μm, 120Å, 2.1x150mm | 1 |
| SLHC8U321200 | C8-Universal, 3μm, 120Å, 2.1x200mm | 1 |
| SLHC8U321250 | C8-Universal, 3μm, 120Å, 2.1x250mm | 1 |
| SLHC8U33030 | C8-Universal, 3μm, 120Å, 3.0x30mm | 1 |
| SLHC8U33050 | C8-Universal, 3μm, 120Å, 3.0x50mm | 1 |
| SLHC8U33075 | C8-Universal, 3μm, 120Å, 3.0x75mm | 1 |
| SLHC8U330100 | C8-Universal, 3μm, 120Å, 3.0x100mm | 1 |
| SLHC8U330125 | C8-Universal, 3μm, 120Å, 3.0x125mm | 1 |
| SLHC8U330150 | C8-Universal, 3μm, 120Å, 3.0x150mm | 1 |
| SLHC8U330200 | C8-Universal, 3μm, 120Å, 3.0x200mm | 1 |
| SLHC8U330250 | C8-Universal, 3μm, 120Å, 3.0x250mm | 1 |
| SLHC8U34040 | C8-Universal, 3μm, 120Å, 4.0x30mm | 1 |
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