C8-Universal HPLC Columns

Wavelength: 220nm
Temp.: Ambient
Flow Rate: 1.0mL/min
Injection Volume: 5μL

Description

C8 HPLC Column Description

The C8 HPLC column is based on ultra-pure silica gel for a thorough double-end bonding process.
C8 column has classical reverse-phase retention and selectivity that is slightly less than the C18 column retention capacity and is therefore recommended for separation on highly hydrophobic compounds and LC/MS instruments. C8 requires less analysis time than C18 when neutralizing neutral or other compounds with strong retention under the same chromatographic conditions.
1. A liquid phase analytical column with a slightly weaker retention capacity than C18
2. Wide pH tolerance range: 1.5-10
3. Peak shape, super high efficiency

C8 HPLC Column Parameters

Non-polar HPLC column
Bonded phase: C8 (USP L7)
Particle size: 3μm, 5μm, 10μm
Aperture: 120Å
Specific surface area: 320m²/g (120Å)
Carbon loading: 12% (120Å)
Sealing: Double tailing
pH: value stability 1.5-10.0

Applications

The C8 HPLC column is more suitable for analyzing macromolecular substances, such as some globulins, etc., which are used in conjunction with C8 and a buffer salt eluent.

New column activation

Rinsing  with 80% methanol 0.5ml/min for 4 hours, then change the liquid to analyze the flow phase balance;
If the mobile phase contains buffer salts, please use the transitional mobile phase transition and then analyze the mobile phase balance.

Technical Data

Bonded PhaseUSP NoParticle size (μm)Pore sizeSurface Area (m²/g)Carbon
Loading
PH RangeEnd-capped
C8-UniversalL71.8 µm,3 µm, 5µm,10 µm120Å,
300Å
120Å –320;
300Å–90
12%1.5-10Yes

Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHC8U32130HPLC Column, C8-Universal, 3μm, 120Å, 2.1x30mm1
SLHC8U32133HPLC Column, C8-Universal, 3μm, 120Å, 2.1x33mm1
SLHC8U32150HPLC Column, C8-Universal, 3μm, 120Å, 2.1x50mm1
SLHC8U32175HPLC Column, C8-Universal, 3μm, 120Å, 2.1x75mm1
SLHC8U321100HPLC Column, C8-Universal, 3μm, 120Å, 2.1x100mm1
SLHC8U321125HPLC Column, C8-Universal, 3μm, 120Å, 2.1x125mm1
SLHC8U321150HPLC Column, C8-Universal, 3μm, 120Å, 2.1x150mm1
SLHC8U321200HPLC Column, C8-Universal, 3μm, 120Å, 2.1x200mm1
SLHC8U321250HPLC Column, C8-Universal, 3μm, 120Å, 2.1x250mm1
SLHC8U33030HPLC Column, C8-Universal, 3μm, 120Å, 3.0x30mm1
SLHC8U33050HPLC Column, C8-Universal, 3μm, 120Å, 3.0x50mm1
SLHC8U33075HPLC Column, C8-Universal, 3μm, 120Å, 3.0x75mm1
SLHC8U330100HPLC Column, C8-Universal, 3μm, 120Å, 3.0x100mm1
SLHC8U330125HPLC Column, C8-Universal, 3μm, 120Å, 3.0x125mm1
SLHC8U330150HPLC Column, C8-Universal, 3μm, 120Å, 3.0x150mm1
SLHC8U330200HPLC Column, C8-Universal, 3μm, 120Å, 3.0x200mm1
SLHC8U330250HPLC Column, C8-Universal, 3μm, 120Å, 3.0x250mm1
SLHC8U34040HPLC Column, C8-Universal, 3μm, 120Å, 4.0x30mm1
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