C1 HPLC Columns

1. The reversed-phase mode can be used for separation of hydrophobic peptide and protein
2. Normal-phase mode can be used for separation of high polar compounds


Description of C1 HPLC Columns

The C1 liquid chromatography column is also called a trimethylsilane bonded silica column. The column uses ultra-pure silica gel as the matrix. It is suitable for samples that are hydrophilic, highly polar, and difficult to separate with general reversed-phase or normal-phase HPLC columns. It can be used for the separation of hydrophobic peptides and proteins in reversed phase mode, and for the separation of more polar compounds in normal phase mode.

C1 HPLC columns are made of ultra-pure silica gel, unique stationary phase bonding technology. High column efficiency, good peak shape symmetry, wide pH range.

Characteristics of C1 HPLC Columns

Hydrophobic interaction forces dominate and remain lower than other reverse phase HPLC columns;
It can elute highly hydrophobic compounds in a short time;
Better separation of hydrophilic compounds than other reversed-phase columns under suitable mobile phase conditions;
It is suitable for separating samples with strong polarity and general reverse phase column or normal phase column.


Bonded phase: C1 (USP L13)
Particle size: 3μm, 5μm
Aperture: 120Å,
Specific surface area: 320m²/g (120Å)
Carbon loading: 4% (120Å)
Sealing: Double tailing
PH stability: 1.5-10.0

New column activation

Rinse with 80% methanol 0.5ml/min for 4 hours, then change to analyze mobile phase equilibrium; if mobile phase;
Contains buffer salts, please use the transitional mobile phase transition and then analyze the mobile phase balance.

Technical Data

Bonded PhaseUSP NoParticle size (μm)Pore sizeSurface Area (m2/g)Carbon
pH RangeEnd-capped
C1L135μm120Å3204% 1.5-10Yes

Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHC1546250HPLC Column, C1, 5μm, 120Å, 4.6x250mm1
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