Description of SCX HPLC column
The SCX HPLC column(Strong Cation Exchange) is a kind of high-efficiency ion-exchange HPLC column. It’s a typical cation exchange column using a silica gel matrix with an aromatic sulfonic acid group as the bonding phase. It is commonly used for the separation of basic, water-soluble compounds. Specific applications include organic bases such as basic amino acids, anilines, pharmaceutically acceptable salts, inorganic cations, and nucleotides.
The bonded stationary phase is a strong cation exchange packing prepared by using high-purity silica gel with good mechanical stability as a matrix and using high-purity bonding reagents, with a mixed chemical structure of sulfonic acid groups and phenyl functional groups. Adopts a unique and innovative design, which can maximize the coverage of single-layer functional groups. Because its surface coverage has been maximized, it has excellent stability.
Organic modifiers can be used on SCX columns to control sample retention time by changing pH, ionic strength, and organic modifier content to provide high efficiency and fast analysis
Technical Parameters of SCX Column
- Particle size: 5μm
- Aperture: 120Å
- Specific surface area: 320m²/g
- Carbon loading: /
- pH range: 2-8
- Whether to seal: No
Applications of SCX HPLC Column
- Adopt highly controllable polymerization monolayer formation and tail sealing technology
- Mixed chemical structure containing sulfonic acid group and phenyl functional group
- It has dual functions of ion exchange and hydrophobic effect, so it has high selectivity and suitable retention for many compounds
- Due to the use of polymer chemical bonding and end-sealing technology, it has excellent stability
- Suitable for the separation of cations, nitrogen, and neutral compounds
- Use guard column and pre-column to extend the service life of the chromatographic column;
- After using for a period of time (about one week), it is necessary to backwash the column with pure methanol (or acetonitrile) at 1.0ml/min for 90min. Pay attention to the transition during the washing process to prevent the precipitation of buffer salts.