Description of SCX HPLC columns
The SCX HPLC column(Strong Cation Exchange) is a kind of high-efficiency ion exchange HPLC column. It’s a typical cation exchange column using a silica gel matrix with an aromatic sulfonic acid group as the bonding phase. It is commonly used for the separation of basic, water-soluble compounds. Specific applications include organic bases such as basic amino acids, anilines, pharmaceutically acceptable salts, inorganic cations, and nucleotides.
The bonded stationary phase is a strong cation exchange packing prepared by using high-purity silica gel with good mechanical stability as a matrix and using high-purity bonding reagents, with a mixed chemical structure of sulfonic acid groups and phenyl functional groups.
The bonded phase adopts a unique and innovative design, which can maximize the coverage of single-layer functional groups. Because its surface coverage has been maximized, it has excellent stability
Organic Modifiers Can be Used on SCX Columns
Control sample retention time by changing pH, ionic strength and organic modifier content
pH range is 2.0-8.0
Provide high efficiency and fast analysis
Technical Parameters of SCX Columns
Particle size: 5μm
Specific surface area: 320m²/g
Carbon loading: /
pH range: 2-8
Whether to seal: No
1) Use guard column and pre-column to extend the service life of chromatographic column;
2) After using for a period of time (about one week), it is necessary to backwash the column with pure methanol (or acetonitrile) at 1.0ml/min for 90min. Pay attention to the transition during the washing process to prevent the precipitation of buffer salts.