C8 Low pH HPLC Columns

Wavelength: 285nm
Temperature: 40°C
Flow Rate: 1mL / min
Injection Volume: 20μL
Notes: After every injection, need 10 column volume to get gradient equilibrium

Description

C8 Low pH HPLC Column Description

The C8 low pH HPLC column is a low pH octylsilyl silica gel HPLC column, it’s particularly suitable for the separation of polarisations from very low to moderate pH. The pH range (0.5-8.0) is used. The LP-C8 uses a special surface treatment and stationary phase construction process. It is protected from hydrolysis at low pH and does not seal at the same time. It avoids the problem of changing the selectivity of C8 due to the easy hydrolysis of the capping reagent under acidic conditions, which makes it have excellent separation performance and stability under acidic mobile phase conditions. Sex and a longer lifespan for the determination of peptide and protein separation under acidic conditions.

C8 Low pH HPLC Column Characteristics

Ultra-pure, fully porous spherical silica gel with a purity of > 99.999%
HPLC column for protein analysis
Unique stationary phase bonding
High efficiency: theoretical plate number > 90000/m
Good peak shape symmetry: the tailing factor is controlled at 0.95-1.05
Wide pH range: suitable for pH 1.0-8.0
Long service life
Excellent batch and batch reproducibility
Resistant 100% aqueous phase
Strong retention capacity for strong polar compounds
The same bonding process with C18-fluorine, the same characteristics with C8-fluorine
Classic reversed phase retention and selectivity that is a little smaller than C8-fluorine
Provide 300Å pore size

C8 Low pH HPLC Column Parameters

Bonded phase: C8 (USP L8)
Particle size: 3μm, 5μm, 10μm,
Aperture: 120Å
Specific surface area 320m²/g (120Å)
Carbon loading: 5.5% (120Å)
Sealing: Unfinished
pH stability: 1.0-8.0

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Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHC8L32130HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x30mm1
SLHC8L32133HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x33mm1
SLHC8L32150HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x50mm1
SLHC8L32175HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x75mm1
SLHC8L321100HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x100mm1
SLHC8L321125HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x125mm1
SLHC8L321150HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x150mm1
SLHC8L321200HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x200mm1
SLHC8L321250HPLC Column, C8-Low pH, 3μm, 120Å, 2.1x250mm1
SLHC8L33030HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x30mm1
SLHC8L33050HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x50mm1
SLHC8L33075HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x75mm1
SLHC8L330100HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x100mm1
SLHC8L330125HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x125mm1
SLHC8L330150HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x150mm1
SLHC8L330200HPLC Column, C8-Low pH, 3μm, 120Å, 3.0x200mm1
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