HPLC Chromatography Column
HPLC (High Performance Liquid Chromatography) columns are the core components of HPLC systems, responsible for separating different components in a mixture. The column is filled with a stationary phase material, most commonly a silica matrix, which can react chemically or physically with the sample components in different ways. Depending on the separation mechanism, columns can be divided into reverse phase columns (using non-polar stationary phases) and normal phase columns (using polar stationary phases), as well as ion exchange columns. During the analysis process, the sample is injected into the column through the action of the mobile phase (usually a liquid solvent). As the sample moves through the column, the different components interact with the stationary phase at different forces, resulting in different residence times (retention times) in the column, and finally eluted from the column in sequence to achieve separation.
HPLC columns come in various sizes, and the length and inner diameter determine the separation effect and the length of the analysis time. They are widely used in fields such as drug analysis, environmental testing, and food quality control, and can efficiently separate complex samples and obtain highly sensitive and high-resolution results.

HPLC columns’ length and inner diameter
Hawach Scientific Co., Ltd is a professional HPLC chromatography column supplier. Hawach HPLC columns include amino acid, C18 Low PH, C18 Aqueous, C8 Universal, C8 Low pH, C8 Aqueous, PAH, Phenyl, NH2, CN, Diol, SiO2, Polar-RP, SCX, etc. Hawach reversed phase (RP) C4 HPLC columns offer an array of RP chemistries to optimize separations and enhance retention or changes in elution order. The NH2 HPLC column uses the innovative filler bonding technology unique to the company.
The following are all chromatographic columns, please choose to buy:
| XChroma Columns | Parameter | ||||||
| Bonded Phase | USP No | Particle Size (μm) | Pore Size | Surface Area (m2/g) | Carbon loading | PH Range | End-capped |
| C18-Aqueous | L1 | 3,5,10 | 120Å | 320 | 12% | 1.5-10 | YES |
| C18-Universal | L1 | 1.8,3,5,10 | 120Å | 320 | 17% | 1.5-10 | YES |
| 300Å | 90 | 8% | |||||
| C18-Low PH | L1 | 5 | 130Å | 160 | 10% | 2.0-8.0 | NO |
| C18-Alkaline | L1 | 3,5,10 | 120Å | 320 | 17% | 1.5-10 | NO |
| C8-Universal | L7 | 1.8,3,5,10 | 120Å | 320 | 12% | 1.5-10 | YES |
| 300Å | 90 | ||||||
| C8-Low PH | L7 | 5 | 120Å | 320 | 5.5% | 1.0-8.0 | NO |
| C8-Fluorine | L7 | 5 | 120Å | 320 | 12% | 1.5-10 | YES |
| C1 | L13 | 5 | 120Å | 320 | 4% | 1.5-10 | YES |
| C4 | L26 | 3,5 | 120Å | 320 | 8% | 1.5-10 | YES |
| 300Å | 90 | 3% | |||||
| SiO2 | L3 | 5 | 120Å | 320 | N/A | 1.5-10 | NO |
| NH2 | L8 | 5 | 120Å | 320 | N/A | 1.5-10 | NO |
| CN | L10 | 5 | 120Å | 320 | 6% | 1.5-10 | NO |
| Phenyl | L11 | 3,5,10 | 120Å | 320 | 12% | 1.5-10 | YES |
| 300Å | 90 | 6% | |||||
| Phenyl-Ether | / | 5 | 120Å | 320 | 12% | 1.5-10 | YES |
| Polar RP | L1 | 1.8,3,5,10 | 120Å | 320 | 18% | 1.5-10 | YES |
| 300Å | 90 | ||||||
| SCX | / | 5 | 120Å | 320 | / | 2.0-8.0 | NO |
| SAX | / | 5 | 120Å | 320 | 7.5% | 2.0-8.0 | NO |
| Diol | / | 5 | 120Å | 320 | 2.5% | 1.5-10 | NO |
| C30 | L62 | 5 | 120Å | 320 | 17% | 1.5-10 | YES |
| Amino Acid | / | 3, 5 | 120Å | 320 | 17% | 1.5-10 | YES |
| PAH | / | 5 | 120Å | 320 | 22% | 1.5-10 | YES |
| PFP | / | 5 | 120Å | 320 | 13% | 1.5-10 | YES |
| HILIC Amide | / | 5 | 120Å | 320 | 7% | 2.0-8.0 | NO |






































