Description of C30 HPLC Column
The C30 column for HPLC is a specially designed column for the separation of carotenoid isomers. C30 HPLC column can cis and trans isomers of β-carotene and polar lutein in lutein under the same mobile phase. The isomer and zeaxanthin are separated to separate carotenoids from blood samples, foods, and natural product extracts and can be separated by commercial preparation.
For the separation of hydrophobic and structurally related isomers, the Hawach C30 HPLC reversed-phase column can achieve excellent results. The covalent modification of C30 alkyl silane provides high shape selectivity for ultra-pure, spherical, porous silica columns. They are fully compatible with various aqueous buffers and can be used for a wide range of analyte applications, providing more flexible method development.
- C30 long chain has excellent lipophilic and hydrophobic forces;
- Can also be used to separate fat-soluble substances such as vitamin E;
- The recommended pH application range is 2.0-6.0.
The long chain has excellent lipophilic and hydrophobic properties and can also be used to separate vitamins.
Analysis of fat-soluble substances.
For neutral and hydrophobic compounds, the retention characteristics of the C30 column are similar to the universal C18 column, but C30 can separate α-, β-, γ- and δ- vitamin E, which is difficult for ordinary C18 columns.
C30 HPLC Column Characteristics
- The separation advantage of C30 long chain bonding relative to the carotenoid isomer column;
- The cis, trans-β-carotene and the polar lutein isomers of the lutein and zeaxanthin in red can be separated under the same mobile phase conditions;
- Resolves polar and non-polar carrot geometries;
- Blood samples, foods, and natural product extracts can be separated.
- Bonded phase: C30 (USP: L62)
- Particle size: 3μm, 5μm, 10μm
- Aperture: 120Å
- Specific surface area: 320m²/g (120Å)
- Carbon loading: 17% (120Å)
- Sealing: Double tailing
- pH stability: 1.5-10.0