C30 HPLC Column

  1. Polymeric C30 alkyl chains
  2. Very lipophilic
  3. Exceptional selectivity pattern for geometric isomers

Availability: In Stock


Description of C30 HPLC Column

The C30 column for HPLC is a specially designed column for the separation of carotenoid isomers. C30 HPLC column can cis and trans isomers of β-carotene and polar lutein in lutein under the same mobile phase. The isomer and zeaxanthin are separated to separate carotenoids from blood samples, foods, and natural product extracts and can be separated by commercial preparation.

For the separation of hydrophobic and structurally related isomers, the Hawach C30 HPLC reversed-phase column can achieve excellent results. The covalent modification of C30 alkyl silane provides high shape selectivity for ultra-pure, spherical, porous silica columns. They are fully compatible with various aqueous buffers and can be used for a wide range of analyte applications, providing more flexible method development.

  1. C30 long chain has excellent lipophilic and hydrophobic forces;
  2. Can also be used to separate fat-soluble substances such as vitamin E;
  3. The recommended pH application range is 2.0-6.0.

The long chain has excellent lipophilic and hydrophobic properties and can also be used to separate vitamins.
Analysis of fat-soluble substances.

For neutral and hydrophobic compounds, the retention characteristics of the C30 column are similar to the universal C18 column, but C30 can separate α-, β-, γ- and δ- vitamin E, which is difficult for ordinary C18 columns.

C30 HPLC Column Characteristics

  • The separation advantage of C30 long chain bonding relative to the carotenoid isomer column;
  • The cis, trans-β-carotene and the polar lutein isomers of the lutein and zeaxanthin in red can be separated under the same mobile phase conditions;
  • Resolves polar and non-polar carrot geometries;
  • Blood samples, foods, and natural product extracts can be separated.


  • Bonded phase: C30 (USP: L62)
  • Particle size: 3μm, 5μm, 10μm
  • Aperture: 120Å
  • Specific surface area: 320m²/g (120Å)
  • Carbon loading: 17% (120Å)
  • Sealing: Double tailing
  • pH stability: 1.5-10.0

Technical Data

Bonded PhaseUSP NoParticle size (μm)Pore sizeSurface Area (m2/g)Carbon
pH RangeEnd-capped

Ordering Information

Item NoDescriptionPackage (pcs/pk)
SLHC3032130C30, 3μm, 120Å, 2.1x30mm1
SLHC3032133C30, 3μm, 120Å, 2.1x33mm1
SLHC3032150C30, 3μm, 120Å, 2.1x50mm1
SLHC3032175C30, 3μm, 120Å, 2.1x75mm1
SLHC30321100C30, 3μm, 120Å, 2.1x100mm1
SLHC30321125C30, 3μm, 120Å, 2.1x125mm1
SLHC30321150C30, 3μm, 120Å, 2.1x150mm1
SLHC30321200C30, 3μm, 120Å, 2.1x200mm1
SLHC30321250C30, 3μm, 120Å, 2.1x250mm1
SLHC3033030C30, 3μm, 120Å, 3.0x30mm1
SLHC3033050C30, 3μm, 120Å, 3.0x50mm1
SLHC3033075C30, 3μm, 120Å, 3.0x75mm1
SLHC30330100C30, 3μm, 120Å, 3.0x100mm1
SLHC30330125C30, 3μm, 120Å, 3.0x125mm1
SLHC30330150C30, 3μm, 120Å, 3.0x150mm1
SLHC30330200C30, 3μm, 120Å, 3.0x200mm1
SLHC30330250C30, 3μm, 120Å, 3.0x250mm1
SLHC3034030C30, 3μm, 120Å, 4.0x30mm1
SLHC3034050C30, 3μm, 120Å, 4.0x50mm1
SLHC3034075C30, 3μm, 120Å, 4.0x75mm1
SLHC30340100C30, 3μm, 120Å, 4.0x100mm1
SLHC30340125C30, 3μm, 120Å, 4.0x125mm1
SLHC30339150C30, 3μm, 120Å, 3.9x150mm1
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