Wide Application of HPLC Columns
HPLC columns( such as C8 Low pH HPLC Columns, PAH HPLC Column, C18 HPLC Columns ) are the most important part, usually made of thick-walled glass tubes or stainless steel tubes with polished inner walls. Copper, aluminum, or Teflon tubes are available for corrosive samples requiring high-pressure resistance.
HPLC is more suitable for the separation and analysis of substances with a high boiling point, poor thermal stability, physiological activity, and relatively large molecular weight.
Hawach HPLC columns are widely used in the analysis of nucleic acid, peptides, lactones, polycyclic aromatic hydrocarbons, polymers, drugs, human metabolites, surface active agents, antioxidants, pesticides, herbicides analysis, and so on. Its instrument structure and flow are various.
HPLC requires only that the sample can be made into a solution without gasification, so it is not limited by the volatility of the sample. According to statistics, about 20% of the known compounds can be analyzed by gas chromatography, and about 70-80% can be analyzed by liquid chromatography. Since 1990, with the rapid development of bioengineering and life science at the international and domestic, HPLC technology is proposed the more updated subject of separation, purification, and preparation, such as the human genome project, proteomics, and so on.
Wide Application of HPLC Columns
- Pharmaceutical Analysis: HPLC columns play a vital role in pharmaceutical analysis for drug discovery, development, and quality control. They are used to separate and quantify active pharmaceutical ingredients (APIs), impurities, degradation products, and metabolites in drug formulations.
- Environmental Analysis: HPLC columns are widely used in environmental analysis to detect and quantify various contaminants, such as pesticides, herbicides, pollutants, and toxins in water, soil, air, and other environmental samples.
- Food and Beverage Analysis: HPLC columns are employed in the analysis of food and beverage products to determine the presence and concentration of additives, preservatives, natural compounds, contaminants, and other components. This includes analyzing vitamins, amino acids, sugars, flavor compounds, and food additives.
- Forensic Analysis: HPLC columns are utilized in forensic laboratories for the analysis of drugs of abuse, toxicology screening, identification of controlled substances, and the detection of trace compounds in forensic samples.
- Biochemical and Biopharmaceutical Analysis: HPLC columns are commonly used in the analysis of biomolecules, including proteins, peptides, nucleic acids, carbohydrates, and metabolites. They are essential in bioanalysis, proteomics, genomics, and biopharmaceutical characterization.
- Clinical Diagnostics: HPLC columns are employed in clinical laboratories for the analysis of clinical samples, such as blood, urine, serum, and plasma. They are used to measure analytes, including drugs, hormones, vitamins, and other compounds relevant to diagnosing diseases and monitoring patient health.
- Chemical Research and Synthesis: HPLC columns are valuable tools in chemical research and synthesis for the purification and isolation of compounds, reaction monitoring, and product characterization. They aid in the separation and identification of reaction components and byproducts.
- Polymer Analysis: HPLC columns are utilized in polymer analysis for the determination of molecular weight distribution, polymer composition, and the analysis of additives, monomers, and degradation products in polymer samples.
- Natural Product Analysis: HPLC columns are extensively employed in the analysis of natural products, such as herbal extracts, essential oils, phytochemicals, and natural compounds. They assist in the identification, quantification, and characterization of bioactive constituents.