Compared with classical liquid chromatography, HPLC has the following advantages:
Fast – usually analyze one sample at 15~30 min, and some samples can be completed in 5 min.
High-sensitivity – UV detectors are up to 0.01 ng, fluorescence and electrochemical detectors up to 0. 1pg.
Columns can be used repeatedly – separate columns can be used to separate different compounds.
A small amount of sample, easy to recycle – the sample can be collected without being destroyed after passing through the column, and a single component can be collected or prepared.