High-performance liquid chromatography (HPLC) was developed on the basis of classical liquid chromatography and introduced gas chromatography theory in the late 1960s. It differs from classical liquid chromatography in that the filler particles are small and uniform, and the small particles have high column efficiency, but cause enthalpy resistance.
The mobile phase needs to be transported by high pressure, so it is also called High-Pressure Liquid Chromatography(HPLC). It is also called High-Speed Liquid Chromatography (HSLP) because of its fast analysis speed. It is also known as modern liquid chromatography.
Advantages Compared with classical liquid chromatography, HPLC has the following advantages: Fast – usually analyze one sample at 15~30 min, and some samples can be completed in 5 min. High-sensitivity – UV detectors are up to 0.01 ng, fluorescence and electrochemical detectors up to 0. 1pg.
Columns can be used repeatedly – separate columns can be used to separate different compounds.
A small amount of sample, easy to recycle – the sample can be collected without being destroyed after passing through the column, and a single component can be collected or prepared.