Precautions For Using Buffer
The first aspect that should also pay attention to while using an HPLC reversed-phase column is to avoid the use of hydrochloride, which is corrosive to steel. Secondly, it prefers to prepare the buffer solution when it is needed. Usually, the buffer solution is the fungus culture solution, which indicates that there would be some strange phenomena if the experiment is conducted in the next day or a long time.
It is necessary to know the pH range in time. During the long term use of buffer solution, it is essential to observe whether there is any precipitation. If there is any white salt precipitated, the 10% nitric acid flushing liquid can be used regularly to avoid blockage.