Three Things to Pay Attention to in C18 HPLC Columns
Hawach will introduce you to three things you should pay attention to when using C18 HPLC columns.
1. Because the stationary phase of C18 HPLC columns is highly hydrophobic, try not to use high water phase conditions during use. If the water is too high, it will cause the stationary phase to collide, causing a rapid decline of column efficiency and even cause Irreversible negative impact; due to the high carbon loading, the performance is particularly obvious, it is recommended that the water ratio in the process of use does not exceed 90%;
2. C18 liquid chromatography column pressure rise, column performance decline: usually, the column is contaminated, the column can be regenerated to restore part of the efficiency;
3.C18 HPLC columns pressure swell: usually caused by salt precipitation or sieve plate blockage, if the salt is precipitated, rinse with a low flow rate of 10% methanol-water until the pressure is restored; if it is judged that salt is not precipitated, pure methanol or 10% Methanol water is the opposite flow column (reverse the column, not connected to the detector). If there is no effect after recoil for half an hour, the recoil is invalid, and other reasons need to be found.