The Principle and Cleaning Method of NH2 HPLC Columns
NH2 HPLC columns refer to the chromatographic columns that use silica gel as matrix carrier to bond amino groups. They are usually used for the analysis of carbohydrate compounds, and the mobile phase is usually methanol, acetonitrile and buffers salt solution.
The cleaning method of NH2 HPLC columns
Foe columns used under positive phase condition, refer to the cleaning method of silica gel columns. For columns used under reverse phase condition, refer to the cleaning method of C18 HPLC columns.
The principle of NH2 HPLC columns
Based on RP principle.
The separation of component molecules on the amino column mainly depends on the hydrogen bond force, and the orientation force and the induction force in the van der Waals force.
Regeneration of NH2 HPLC columns
Under abnormal circumstances, a series of remedial measures are taken to improve the column efficiency and prolong the service life.
NH2 HPLC columns are mainly used to separate optical isomers and substances with the large polar proportion that can’t be separated by reversed phase column, and analyze monosaccharides and hydrocarbon compounds with the features of weak retention, high polarity, unstable, easy hydrolysis under acidic conditions.