What is the Chromatographic Mode of an R-P HPLC Column?
The reversed-phase high-performance liquid chromatography column is a chromatographic mode based on the hydrophobic effect between the solute, the polar mobile phase and the surface of the non-polar stationary phase. Any organic molecule has a non-polar hydrophobic portion in its structure. The larger the part, the higher the general retention value. In high-performance liquid chromatography, this is a widely used separation mode. Under the condition of reversed-phase liquid chromatography of biological macromolecules, the mobile phase is mostly acidic and low. An aqueous solution of ionic strength, plus a certain proportion of organic modifiers such as isopropanol, acetonitrile or methanol which are miscible with water, and a large amount of filler is a silica alkyl bonded phase having a pore diameter of 30 nm or more. There are also a small amount of high polymer microspheres.
In the daily separation and analysis work, the correct use and maintenance of the RP-HPLC column are very important. If the column is used properly, it will directly affect the life of the column. If it is inadvertently, it will reduce efficiency, shorten the service life and even damage it. During the chromatographic operation, there are some issues to be aware of to maintain the column.