The Basic Knowledge Of HPLC Column

HPLC column is a separate analysis technology developed in the late 1960s and is an important means of modern separation and determination. It is widely used in biochemistry, drug, and clinical analysis because of its high separation efficiency, fast analysis speed, good detection sensitivity, and ability to analyze high-boiling but not vaporizable thermo-labile physiologically active substances.


The system of high-performance liquid chromatography (HPLC) column consists of a reservoir, a pump, an injector, a column, a detector, a recorder, and the like.

Working principle

The high-pressure pump drives the mobile phase in the reservoir into the system and the sample solution enters the mobile phase via the sample injector and is flowed to the stationary phase.

Since the components in the sample solution have different partition coefficients in the two phases, each component produces a large difference in moving speed, after repeated multiple adsorption-desorption processes, during the relative motion in the two phases.

Thus they are separated into individual components and sequentially flowed out of the column. When passing through the detector, the sample concentration is converted into an electrical signal and transmitted to the recorder, and the data is printed in the form of a graph.