Storage Conditions and Separation Effect for HPLC Column

Storage Conditions for HPLC Column

Hawach HPLC columns are individually packed and tested to guarantee outstanding quality and performance. Storage conditions of the HPLC column greatly determine the column lifetime, so it is significant to store columns in good conditions.

There are some tips for it: never store HPLC columns with iron-pairing or buffers reagent; there is no need to use buffer to remove any buffers or salts when flashing columns. Considering interactions between sample components and stationary phase, Hawach HPLC column uses optimum conditions to decrease band-spreading from extra HPLC column effects.

Separation Effect for HPLC Column

The separation effect of the chromatographic column depends on the selected stationary phase and the preparation and operation conditions of the chromatographic column. Chromatography is a means of separation and analysis, separation is the core, so the column responsible for separation is the heart of the chromatographic system.

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In order to improve the column efficiency and reduce the tube wall effect, the inner wall of the stainless steel column is polished. Some people apply fluorine plastic to the inner wall of the stainless steel column to improve the finish of the inner wall, which is the same as polishing.

Chromatographic column washing, according to different types of columns, sample properties to specific washing, the situation is more complex. Read the instructions before use, can communicate with the chromatographic column supplier technical personnel, how to use. When washing, it is better to pay attention to the pressure, preferably from the small flow rate to slowly increase the flow rate to the pressure permitted range. The time of washing, everyone has a misunderstanding is that the washing time in minutes or hours is inaccurate, the accuracy is that the flow of general chromatographic column washing should reach 10-20 times the column volume.

Stationary Phase of HPLC Column

In chromatography, a stationary phase (solid or liquid) is called a stationary phase; a moving phase (usually a gas or liquid) is called a mobile phase. The column chromatography is to fill the stationary phase into the glass tube, pour the solution to be separated above with the mobile phase solvent infiltration, and then add the mobile phase because the adsorption force of the stationary phase is different, the adsorption force is fixed or moving slowly.

Thin-layer chromatography is coated on the glass plate with a stationary phase coating, then spot, the lower end immersed in the solvent, the same bottom-up separation. It is often used to explore the experimental conditions of column chromatography, the selection of solvent, and the stationary phase.