Precautions During The Use Of RP-HPLC Columns
When storing the column, the column should be filled with acetonitrile or methanol, and the column joint should be tightened to prevent the solvent from evaporating and drying. It is absolutely forbidden to leave the buffer solution in the HPLC columns for an overnight or longer period of time.
During the use of reversed-phase high-performance liquid chromatography HPLC column, if the pressure rises, one may be that the sintered filter is clogged, then the filter should be replaced or taken out for cleaning; the other may be macromolecule In the column of the human column, the stigma is contaminated; if the efficiency of the column is reduced or the peak of the chromatogram is deformed, the stigma may collapse and the dead volume increases.
The HPLC columns are often washed with a strong solvent to remove impurities remaining in the column. When cleaning, the displacement of the mobile phase in the flow path system should be gradually transitioned with a solvent that is miscible. The volume of each mobile phase should be about 20 times the volume of the column, that is, 50-75 mL is required for routine analysis.