Polyphenols in Tobacco Samples By HPLC Column

The determination of 10 plant polyphenols in tobacco samples by solid phase extraction and high-performance liquid chromatography as below:

Plant polyphenols are important substances in tobacco. The content of polyphenols in tobacco has an important influence on tobacco quality. Therefore, it is of great significance to study the plant polyphenols in tobacco and its products. The determination methods of tobacco polyphenols mainly include spectrophotometry, gas chromatography, and liquid chromatography. The photometric method has poor accuracy and can only approximately determine the total amount.

Gas chromatography requires complex derivatization; The traditional liquid chromatography requires the sample to be decreased with the organic solvent, which is difficult to operate and will cause polyphenol loss. In recent years, solid phase extraction (SPE) has been widely used in analytical chemistry.

The SPE Cartridge C18 was used for pre-separation and degreasing, and HPLC Column(3. 9 mm x 150 mm) was used for separation and determination of polyphenols in tobacco. The main polyphenols were characterized by UV spectrogram. The method can simultaneously determine 10 kinds of polyphenols in tobacco and be used for tobacco sample analysis.