Operation And Tips For Management of HPLC Columns
Operation of HPLC Columns
In daily separation analysis, it is significant to properly use column, or the service life of the column efficiency of it can be directly affected. In the course of column loading, unloading or replacement, strong mechanical vibration should be prevented so as not to produce gaps in the column bed.
If the column is used for routine analysis, a special column may be arranged for each kind of conventional analysis to prolong the life of the column.
Additionally, avoid sharp changes in pressure and temperature, because the sudden temperature will affect the filing condition of the column and urge the packing in the column. And the rotation of the valve should not be too slow when the valve is injected.