Improvements of HPLC Column
HPLC Column is an important separation and analysis technology in chemistry, medicine, industry, agriculture and so on. Aiming at solving the demanding problems occurred on pressure, peak shape and retention, Hawach HPLC column determines the cause and finds methods to overcome it as follows.
For backpressure problems, Hawach uses column protection to guard columns and in-line filters and takes appropriate column flushing and filter buffered mobile phase. Those steps can well prevent back pressure problems. Additionally, peak shape issues such as split peaks, peak tailing, broad peaks, and poor efficiency are greatly avoided in Hawach’s HPLC column.
Management and maintenance of HPLC columns
Correct use and maintenance of PHLC columns are very important, a little carelessness will reduce the column efficiency, shorten the service life and even damage. In the course of HPLC operation, it is necessary to avoid the direct mechanical impact or fall of the chromatographic column and the degradation of the performance of the chromatographic column.
And never store columns with buffers or complex reagents, avoid sharp changes in pressure and temperature and any mechanical vibration. After each work, it is best to rinse with eluent with strong eluting ability.