Inorganic Fillers and Polymer Fillers in HPLC Columns

Hawach will introduce two fillers in the HPLC column from inorganic fillers and polymer fillers, respectively.

Inorganic fillers in HPLC columns

1. FLORISIL magnesium silicate, a highly selective adsorbent for the purification, separation, separation of endocrine and oil, and analysis of pigments;
2. PESTICARD graphitized carbon, a new carbon black material with high purification effect, high recovery rate and high reproducibility, is used for pesticide residue analysis, especially high-quality sample preparation such as vegetables and fruits;
3. Neutral alumina N, PH=7.5, strong polar adsorbent, neutral surface, easy to retain heterocyclic compounds, aromatic hydrocarbons and organic amines and other rich compounds, widely used in the analysis of Sudan red and malachite green;
4. Acidic Alumina A, PH=4.5, can be used as strong polar adsorption and medium cation exchanger;
5. Alkaline alumina B, PH-10, can be used to remove organic acids, phenols, etc.
6. Silica (SiO2): Silica is a common inorganic filler used in HPLC columns. Silica serves as the solid support for the stationary phase. It provides a large surface area for interactions with analytes.

Polymer fillers in HPLC columns:

1. PEP pyruvic acid, a functional polystyrene/divinylbenzene extraction column, has both hydrophilic and lipophilic groups on the surface and has a relatively balanced adsorption effect on polar and non-polar compounds. Widely used in the analysis of various compounds;
2. HXN sulfonylureas, specially prepared for the preparation of sulfonylurea samples, for the extraction, enrichment, and purification of various moderately polar to strong polar compounds;
3. PS polystyrene, unsubstituted polystyrene/divinylbenzene, has a high specific surface area, is slightly more memorable than C18 bonded silica, and has extremely high adsorption and sample capacity for both non-polar and polar compounds;
4. An extraction column of a water-wettable polymer micro matrix of PCX mixed cation, cation exchange mixing mechanism. Provides dual retention modes: ion exchange and reverse phase retention. The filler is stable in the range of PH1-14 and has a large binding capacity;
5. PAX mixed anion: cation exchange mixing mechanism The infiltrating polymer-based extraction column is very stable in PH1-14.

Characteristics and Considerations:

  1. Stationary Phase Composition:
    • Inorganic Fillers: Silica-based columns are common for reversed-phase separations.
    • Polymer Fillers: Polymers like PS-DVB are used for reversed-phase separations, while others like PEG are employed in size exclusion and other specialty chromatographic modes.
  2. Selectivity:
    • Inorganic Fillers: Silica provides different selectivity for compounds based on its surface properties.
    • Polymer Fillers: Polymer-based columns may offer unique selectivity and can be tailored for specific applications.
  3. Pore Size and Surface Area:
    • Inorganic Fillers: Silica columns can have different pore sizes and surface areas, influencing separation efficiency.
    • Polymer Fillers: Pore sizes in polymer-based columns are often controlled to suit specific separation requirements.
  4. Chemical Stability:
    • Inorganic Fillers: Silica is chemically stable under a wide range of conditions but may be prone to hydrolysis under extreme pH.
    • Polymer Fillers: Polymer-based columns are chemically stable and can be used in a broader pH range.
  5. Applications:
    • Inorganic Fillers: Silica is widely used in reversed-phase and normal-phase chromatography.
    • Polymer Fillers: Polymers find applications in various modes, including reversed-phase, ion-exchange, and size exclusion chromatography.