HPLC Column Silicone Matrix Properties and Scope of Application
Hawach introduced the properties and application range of HPLC column stationary phase in the silica gel matrix.
Silica gel matrix:
Silica gel, weakly acidic, has a strong memory, used to separate non-polar, weakly acidic compounds, oils, etc.;
C18, a silica-based uncapped reversed-phase C18 column with high bond and density, low loss, high recovery, etc.
C8 octyl, the retention of non-polar compounds is weaker than C18;
CN Cyano, a silica-based cyanopropyl column with moderate polarity for reversed phase or normal phase separation, suitable for carbohydrates and cationic compounds;
NH2 amino group, silica-based aminopropyl stationary phase, used for the separation of phenol and phenolic pigments, petroleum hexaphenol, sugars, drugs and metabolites;
PSA-propyl ethylenediamine, a stationary phase similar to NH2, PSA has two amino groups, which is more ion exchangeable than NH2, and PSA can also chelate with metal ions for extracting metal ions;
SAX strong anion exchange, bonded with quaternary ammonium salt functional groups, mainly used for the separation of weak anionic compounds;
COOH carboxyl group, the bonding functional group is a carboxylic acid, used for the separation of quaternary ammonium salts or other strong cations;
PRS propane sulfonic acid, the bonding functional group is a sulfonic acid, used for weak cations, widely used in the analysis of malachite green;
SCX strong cation exchange, bond, and the functional group is benzenesulfonic acid, used for separation of organic base compounds and biological macromolecule desalting.