How to Cleaning of Protein Residues in HPLC Column
Silica matrix HPLC columns are generally compatible, but organic polymer matrix columns may expand or contract in some solvent combinations, which will affect their chromatographic behavior.
Wash each solvent system at least 20 volumes. Because some solvent systems are highly viscous, the flushing flow rate should be adjusted accordingly to ensure that it does not exceed the pump pressure. When the solvent such as guanidine or urine is used up, the HPLC column should be flushed with at least 40 to 50 volumes of HPC water.
Surfactants such as sodium dodecyl sulfonate (SDS) and trinitrotoluene are not recommended for reversed-phase columns because of these modifications.
Compounds are bound to be firmly adsorbed on silica gel bonded phase columns and are difficult to remove. The surfactant can affect the filling surface and change it.
Nature. However, a study by a separation team showed that contaminated columns made by a team could be used with 500 mL 1% SDS.
The solution was scavenged with 1 ml/min flow rate. If we continue to use trifluoride from 5% to 95% containing 1%(v/v)
Gradient elution of acetic acid with ethyl-clear can restore the separation of the polypeptide.