5 Classifications of HPLC Column
Used in analytical chemistry, a high performance liquid chromatography(HPLC) column is the column that can separate out components from the liquid sample. when liquid samples pass through the HPLC column, depending on how they interact with an adsorbent material packed inside the column, analytes elute at different times. For the highest pressure resistance, HPLC columns are usually constructed of stainless steel.
The Hawach HPLC column can not only meet our worldwide customers’needs of fast HPLC separation, but the traditional methods within the pharmaceutical, environmental, clinical, and food industries as well.
The particle platforms which make up the Hawach HPLC columns are monolithic silica, polymeric, monodisperse silica, ultra-pure silica, and zirconia. With the whole set the different products, you can always find the right ones for your application.
Hawach has a tradition of making innovative HPLC Columns with high column efficiency, excellent shape peaks and low pressure for a longer lifetime. And you can find Hawach C18 Universal HPLC column doing the same good performance as Waters, Agilent, and GL Science.
High performance liquid chromatography (HPLC) columns can be roughly divided into five categories.
1.High performance reversed-phase liquid chromatography column
It is extremely widely used in drug metabolism and kinetics, life sciences, medical health, bioanalytical testing, drug, and stimulant testing, food safety analysis, environmental analysis, military, homeland security, etc. It is also one of the most important separations and purification technologies.
2.High efficiency normal-phase liquid chromatography column
It is the earliest chromatographic mode. Until now, further purification through silica gel column after synthesis is still an important part of the daily work of organic chemists. In theory, almost all organic compounds dissolved in n-hexane, ethyl acetate or isopropanol can be analyzed by high performance normal phase liquid chromatography.
However, in practical applications, the application of high efficiency normal-phase liquid chromatography is much less than that of reversed-phase chromatography. This is because n-hexane, ethyl acetate is not as popular as water, methanol or acetonitrile. In addition, A-type silica normal-phase liquid chromatography gives customers inertial thinking: High-efficiency normal-phase liquid chromatography has a long equilibrium time.
3.high efficiency hydrophilic liquid chromatography column
It is a mode of operation between the normal phase and reversed-phase high-performance liquid chromatography. This model was first introduced from the sugar analysis application of the NH2 column. After that, some valuable applications have been found in Diol, silica gel, and hydrophilic polymer-bonded phases.
In recent years, high-performance hydrophilic liquid chromatography has become a popular mode of operation for chromatography, mainly because of the good retention of hydrophilic compounds and the application of high-performance hydrophilic liquid chromatography in LC-MS/MS.
4. high efficiency and strong cation exchange liquid chromatography column
Ion exchange liquid chromatography is one of the most common and useful chromatographic modes for biological separation. On the other hand, it is rarely used for small molecule separation analysis.
5. Innovation high efficiency multi-function liquid chromatography column
We introduce four high-performance multi-function LC columns:
(1) InnovationTMTX Drug Analysis HPLC Column
(2) innovation DNPH HPLC column
(3) InnovationTM PAH HPLC column
(4) Plasma, serum direct injection of InnovationTM PEG column
The InnovationTM PEG column is a versatile column. It is the best normal phase column! At the same time, it can be used as a reverse phase to allow plasma, serum, and urine samples to be injected directly, after using ethanol instead of storage solvent.