5 Classifications of HPLC Column
The HPLC column generally consists of a column tube, a pressure cap, a ferrule (seal ring), a sieve plate (filter), a joint, a screw, and the like. The column is a column tube with a stationary phase for separating the mixture. It is mostly made of metal or glass. It has the shape of a straight tube, a coil, a U-tube, etc., and is widely used in many fields.
Used in analytical chemistry, a high-performance liquid chromatography(HPLC) column is the column that can separate out components from the liquid sample. when liquid samples pass through the HPLC column, depending on how they interact with an adsorbent material packed inside the column, analytes elute at different times. For the highest pressure resistance, HPLC columns are usually constructed of stainless steel.
The Hawach HPLC column can not only meet our worldwide customers’ needs for fast HPLC separation, but the traditional methods within the pharmaceutical, environmental, clinical, and food industries as well.
The particle platforms which make up the Hawach HPLC columns are monolithic silica, polymeric, monodisperse silica, ultra-pure silica, and zirconia. With the whole set the different products, you can always find the right ones for your application.
Hawach has a tradition of making innovative HPLC Columns with high column efficiency, excellent shape peaks, and low pressure for a longer lifetime. And you can find the Hawach HPLC column doing the same good performance as Waters, Agilent, and GL Science.
HPLC column classification
In general, depending on the nature of the sample, which LC method is used, and then select different types of columns. That is, different types of columns represent different chromatographic methods.
The difference between different types of HPLC columns is the difference in column structure, column packing, and column size. Such as NH2 HPLC Column, ODS C18 HPLC Column, C18 Alkaline HPLC Column, and C1 HPLC Column having different column packing.
HPLC columns are available in different sizes (length and inner diameter), sub-preparative, routine analytical, and micro. The hardware of different types of columns is also different (including joints, columns, etc.), as well as radial pressurized columns and jacketed heating columns.
The size of different liquid chromatography methods can be selected according to requirements, and the common analysis is 3 to 30 cm long and the inner diameter is 4 to 8 mm. A column of 20 cm long and 4.6 mm inner diameter is commonly used. The preparation column has an inner diameter of generally 8 mm and a length of 25 cm. The microcolumn has an inner diameter of l to 3 mm and a length of 10 to 20 cm. The effect of different filler analyses may vary, due to differences in the production process. The same type of filler produced by the same manufacturer may vary from batch to batch, and this difference may start from the substrate (surface area, impurities, special treatment), as well as bonded chemicals. Fillers produced by different manufacturers will also vary greatly depending on the patented technology (pretreatment, bonding process, filling technology). Due to various differences, it can only be assumed that the columns of the same batch number have substantially the same properties.
Most column packing matrices use porous silica particles, usually spherical and amorphous, with different particle sizes, pore sizes, and surface areas. Porous polymer microparticles are also suitable for reversed-phase chromatography. The polymer column has a wide mobile phase range and the mobile phase pH can range from 1 to 13. The silica matrix pH can only be between 2.5 and 7. Obviously, the polymer column is better, but it is still dominated by silica-based columns. In principle, polymer columns can overcome some of the deficiencies of silica matrix columns, but a large number of experiments are needed to confirm the overall superiority of polymer matrix fillers.
HPLC columns can be roughly divided into five categories
1. High-performance reversed-phase liquid chromatography column
It is extremely widely used in drug metabolism and kinetics, life sciences, medical health, bioanalytical testing, drug, and stimulant testing, food safety analysis, environmental analysis, military, homeland security, etc. It is also one of the most important separation and purification technologies.
2. High-efficiency normal-phase liquid chromatography column
It is the earliest chromatographic mode. Until now, further purification through silica gel columns after synthesis is still an important part of the daily work of organic chemists. In theory, almost all organic compounds dissolved in n-hexane, ethyl acetate, or isopropanol can be analyzed by high-performance normal-phase liquid chromatography.
However, in practical applications, the application of high-efficiency normal-phase liquid chromatography is much less than that of reversed-phase chromatography. This is because n-hexane and ethyl acetate are not as popular as water, methanol, or acetonitrile. In addition, A-type silica normal-phase liquid chromatography gives customers inertial thinking: High-efficiency normal-phase liquid chromatography has a long equilibrium time.
3. High-efficiency hydrophilic liquid chromatography column
It is a mode of operation between the normal phase and reversed-phase high-performance liquid chromatography. This model was first introduced from the sugar analysis application of the HPLC column. After that, some valuable applications have been found in Diol, silica gel, and hydrophilic polymer-bonded phases.
In recent years, high-performance hydrophilic liquid chromatography has become a popular mode of operation for chromatography, mainly because of the good retention of hydrophilic compounds and the application of high-performance hydrophilic liquid chromatography in LC-MS/MS.
4. High efficiency and strong cation exchange liquid chromatography column
Ion exchange liquid chromatography is one of the most common and useful chromatographic modes for biological separation. On the other hand, it is rarely used for small molecule separation analysis.
5. Innovation of high-efficiency multi-function liquid chromatography column
We introduce four high-performance multi-function LC columns:
(1) InnovationTMTX Drug Analysis HPLC Column
(2) innovation DNPH HPLC column
(3) InnovationTM PAH HPLC column
(4) Plasma, serum direct injection of InnovationTM PEG column
The InnovationTM PEG column is a versatile column. It is the best normal phase column! At the same time, it can be used as a reverse phase to allow plasma, serum, and urine samples to be injected directly, after using ethanol instead of storage solvent.
