FAQ Analysis And Solutions About HPLC Columns
HPLC analysis, which is supposed to have more broader potential applications, is not limited by environment temperature and the boiling point of samples. After more than 30-year rapid development, the theory of HPLC has been more and more mature in the basic theory, the instruments and the chromatography columns, etc. And now HPLC has become one of the most advantageous separation and analysis methods in many fields, such as chemical industry, environment, pharmacy, food and so on.
According to the different separation mechanism, the liquid chromatography can be divided into five kinds: liquid-solid adsorption chromatography, liquid-liquid distribution chromatography, ion-exchange chromatography, ion-pair chromatography and molecular exclusion chromatography, which can be also called gel permeation chromatography.
For instance, in the process of the liquid-solid adsorption chromatography, the mobile phase is liquid and the stationary phase is solid adsorbent. The substances are separated according to the different adsorption effects. And when doing the liquid-liquid chromatography, the mobile phase and stationary phase are both liquid chromatography, which uses the distribution of sample components between two immiscible liquid phases for separation.
FAQ analysis and solutions about HPLC columns
1.The column pressure is too high, after a period of use.
Solution: First check whether the HPLC system is the cause. After eliminating the system cause, the reason is basically due to the accumulation of impurities in the column during the experiment. Try to improve the operating conditions. After the sample is finished, it should be rinsed off in time. For example, the mobile phase of the buffer salt must be washed with a high proportion of aqueous solution (such as 90%) and then stored with the organic phase.