The first prominent feature of the C30 HPLC column is that its chain has excellent lipophilic and hydrophobic forces. Secondly, the recommended pH range for the column is from 2.0 to 6.0. Moreover, its pore size gives it an advantage of a higher flow rate. The stationary phase of the C30 HPLC column provides a unique shape selectivity for structurally related isomers. Meanwhile, it could be compatible with high aqueous mobile phases.
Application of HPLC column
The Diol HPLC column is suitable for both normal phases, reversed-phase and hydro up interaction liquid chromatography (HILIC). At the same time, its surface could be easily wetted by water to form a water-rich layer, which can be used for the analysis and separation of strong polar compounds in HILIC mode. It can be widely used for the analysis of polar natural products, herbicides, metabolites, low sugars, and fats. Diol columns are primarily used for the separation of polar compounds. They are especially well-suited for the analysis of carbohydrates, sugars, alcohols, and other hydrophilic compounds. Diol columns are commonly used in the food and beverage industry for analyzing sugars, sweeteners, and related compounds. Diol columns are commonly used in applications like the analysis of carbohydrates, sugars, glycols, and small polar organic molecules.
The C30 HPLC column can be used to separate fat-soluble substances, such as vitamin E. It is suitable for the separation of the cis and trans isomers of β-carotene and polar lutein isomers in lutein under the same mobile phase. Also, it could be used for the separation of the blood samples and the carotenoids contained natural product extracts. C30 columns are primarily used for the separation of hydrophobic compounds, particularly large nonpolar molecules such as natural products and lipids. They are often employed in pharmaceutical and natural product research. C30 columns are used for the analysis of compounds like natural product extracts, lipids, fatty acids, and other hydrophobic molecules. They are valuable in applications where resolving complex mixtures of nonpolar compounds is required.
In summary, Diol HPLC columns are designed for the separation of polar compounds, especially carbohydrates, and sugars, using hydrophilic interaction chromatography (HILIC). On the other hand, C30 HPLC columns are used for the separation of hydrophobic compounds, particularly large nonpolar molecules, using reversed-phase chromatography. The choice between these columns depends on the specific analytes and separation requirements of your HPLC analysis.