Diol HPLC Column and C30 HPLC Column

Hawach HPLC column has become one of the most important laboratory tools for separation and purification technology, whose main function is to separate each substance in the sample. HPLC technology has been widely used in the fields of chemistry, medicine, food safety, etc. Generally, there are mainly three types of HPLC columns satisfying customers’ various requirements.

By employing the high-purity silica gel as the matrix, the Diol HPLC column uses unique bonding technology to make itself more stable and durable in aqueous media. The detailed information in the column would be described as follows.

Lutein and vitamin E are common nutrient enhancers in foods. The analytical methods for these two types of substances are clearly defined in the food nutrition enhancer use standards. The separation of isomers is also specifically illustrated. The C30 HPLC column is a specially designed column for the separation of carotenoid isomers.

Diol HPLC columns and C30 HPLC columns are two different types of high-performance liquid chromatography (HPLC) columns used for specific applications in analytical chemistry. They have distinct properties and are designed for different types of separations. Here’s an overview of each column:

Diol HPLC Column:

  1. Stationary Phase: Diol columns have a stationary phase made from diol (diol-functionalized silica), which contains hydroxyl (OH) groups.
  2. Selectivity: Diol columns provide a hydrophilic interaction chromatography (HILIC) mode of separation. They can retain and separate polar compounds based on hydrogen bonding and dipole-dipole interactions between the OH groups on the stationary phase and polar functional groups on the analytes.
  3. Mobile Phase: The choice of mobile phase in diol chromatography often includes a mixture of organic solvents (e.g., acetonitrile or methanol) and a polar buffer or water.

C30 HPLC Column:

  1. Stationary Phase: C30 columns have a stationary phase made from C30-bonded silica, typically containing octyl (C8) and octadecyl (C18) groups. The C30 phase contains longer alkyl chains than typical C18 phases.
  2. Selectivity: C30 columns provide reversed-phase chromatography, similar to C18 columns. However, the longer alkyl chains in the C30 phase offer increased hydrophobic interactions, making them suitable for compounds with high hydrophobicity.
  3. Mobile Phase: C30 columns typically use a nonpolar mobile phase, often a mixture of organic solvents (e.g., methanol or acetonitrile) and water.

Features of HPLC column

First of all, the Diol HPLC column has higher selectivity than the normal-phase separated silica gel since the hydrophilicity of the diol group is weaker than the one of pure silica gel. The polarity of its stationary phase is slightly less than the unbonded silica gel, which provides moderate positive phase retention and excellent selectivity. Besides, its high-performance silica gel and special bonding technology could stabilize the diol bonded phase in the aqueous medium without loss of column service life. Also, it has high inertness and durability.

High Quality HPLC Columns
China HPLC Cloumn

The first prominent feature of the C30 HPLC column is that its chain has excellent lipophilic and hydrophobic forces. Secondly, the recommended pH range for the column is from 2.0 to 6.0. Moreover, its pore size gives it an advantage of a higher flow rate. The stationary phase of the C30 HPLC column provides a unique shape selectivity for structurally related isomers. Meanwhile, it could be compatible with high aqueous mobile phases.

Application of HPLC column

The Diol HPLC column is suitable for both normal phases, reversed-phase and hydro up interaction liquid chromatography (HILIC). At the same time, its surface could be easily wetted by water to form a water-rich layer, which can be used for the analysis and separation of strong polar compounds in HILIC mode. It can be widely used for the analysis of polar natural products, herbicides, metabolites, low sugars, and fats. Diol columns are primarily used for the separation of polar compounds. They are especially well-suited for the analysis of carbohydrates, sugars, alcohols, and other hydrophilic compounds. Diol columns are commonly used in the food and beverage industry for analyzing sugars, sweeteners, and related compounds. Diol columns are commonly used in applications like the analysis of carbohydrates, sugars, glycols, and small polar organic molecules.

The C30 HPLC column can be used to separate fat-soluble substances, such as vitamin E. It is suitable for the separation of the cis and trans isomers of β-carotene and polar lutein isomers in lutein under the same mobile phase. Also, it could be used for the separation of the blood samples and the carotenoids contained natural product extracts. C30 columns are primarily used for the separation of hydrophobic compounds, particularly large nonpolar molecules such as natural products and lipids. They are often employed in pharmaceutical and natural product research. C30 columns are used for the analysis of compounds like natural product extracts, lipids, fatty acids, and other hydrophobic molecules. They are valuable in applications where resolving complex mixtures of nonpolar compounds is required.

In summary, Diol HPLC columns are designed for the separation of polar compounds, especially carbohydrates, and sugars, using hydrophilic interaction chromatography (HILIC). On the other hand, C30 HPLC columns are used for the separation of hydrophobic compounds, particularly large nonpolar molecules, using reversed-phase chromatography. The choice between these columns depends on the specific analytes and separation requirements of your HPLC analysis.