Diol HPLC Column and C30 HPLC Column
Hawach HPLC column has become one of the most important laboratory tools for separation and purification technology, whose main function is to separate each substance in the sample. HPLC technology has been widely used in the fields of chemistry, medicine, food safety, etc. Generally, there are mainly three types of HPLC columns satisfying customers’ various requirements.
By employing the high-purity silica gel as the matrix, the Diol HPLC column uses unique bonding technology to make itself more stable and durable in aqueous media. The detailed information of the column would be described as follows.
Lutein and vitamin E are common nutrient enhancers in foods. The analytical methods for these two types of substances are clearly defined in the food nutrition enhancer use standards. The separation of isomers is also specifically illustrated. The C30 HPLC column is a specially designed column for the separation of carotenoid isomers.
Features
First of all, the Diol HPLC column has higher selectivity than the normal-phase separated silica gel, since the hydrophilicity of the diol group is weaker than the one of pure silica gel. The polarity of its stationary phase is slightly less than the unbonded silica gel, which provides moderate positive phase retention and excellent selectivity. Besides, its high-performance silica gel and special bonding technology could stabilize the diol bonded phase in the aqueous medium without loss of column service life. Also, it has high inertness and durability.
The first prominent feature of the C30 HPLC column is that its chain has excellent lipophilic and hydrophobic forces. Secondly, the recommended pH range for the column is from 2.0 to 6.0. Moreover, its pore size gives itself an advantage of a higher flow rate. The stationary phase of the C30 HPLC column provides a unique shape selectivity for structurally related isomers. Meanwhile, it could be compatible with high aqueous mobile phases.
Application
The Diol HPLC column is suitable for both normal phases, reversed-phase and hydro up interaction liquid chromatography (HILIC). At the same time, its surface could be easily wetted by water to form a water-rich layer, which can be used for the analysis and separation of strong polar compounds in HILIC mode. It can be widely used for the analysis of polar natural products, herbicides, and metabolites, low sugars, and fats.
The C30 HPLC column can be used to separate the fat-soluble substances, such as vitamin E. It is suitable for the separation of the cis and trans isomers of β-carotene and polar lutein isomers in lutein under the same mobile phase. Also, it could be used for the separation of the blood samples and the carotenoids contained natural product extracts.
