Chemical Properties of C18 HPLC Columns
Chemical properties of the chromatographic column could affect the performance of the C18 HPLC column.
Performance factors of C18 HPLC columns: bonding type
Single tooth bonding can improve the mass transfer rate and accelerate column balance. Double tooth bonding can increase the stability of the column and increase the sample load.
Performance factors of C18 HPLC columns: carbon coverage
High carbon coverage improves resolution, but analysis takes longer. Low carbon coverage reduces running time.
Performance factors of C18 HPLC columns: sealing the end
The sealing end can reduce the peak dragging phenomenon caused by the reaction between the components to be measured and the residual acid silicon hydroxyl on the surface of the silica gel.
Use of C18 HPLC columns
Before use, it is better to test the performance of the column and save the results as a reference for future evaluation of column performance changes. The performance of the column may vary depending on the sample, mobile phase, column temperature, etc.
Since the column efficiency is a function of the mobile phase velocity in the column, different column efficiency can be obtained by using different velocity.