C18 HPLC Columns Using Guide
The life of the chromatographic column can be directly affected by using routine separation and analysis. In the daily use of C18 HPLC columns, samples and mobile phase were pretreated, but it was still difficult to completely avoid column pollution, fixed phase loss, hardening, column bed collapse, and column efficiency decline after using for a long time.
Renewable technologies of C18 HPLC columns
After contamination, the column can be rinsed with a suitable solvent to regenerate the column efficiency. Regeneration washing method: 60ml of methanol, trichloromethane and methanol/water were respectively used to pass through the chromatographic column successively, followed by 100% methanol (60ml) to balance chromatographic column and then seal.
Repair of C18 HPLC columns
When regeneration is ineffective or column pollution is serious, column repair can be adopted, but the column pollution depth shall not exceed 5mm.
The selection of C18 HPLC columns
1. The alkyl sealing filler can prevent the trailing phenomenon of alkaline compounds.
2. For columns with high carbon content, the retention value is increased.
3. The shorter columns
4. Small particle packing
5. Choose C18 HPLC columns with large pore size packing for the components with large molecular weight.