Application and Characteristics of HPLC Column

Column chromatography is the most primitive chromatographic method, the stationary phase installed in a metal or glass column or fixed phase adsorption on the carrier made of the chromatographic column, the sample from the column head to the end of the column along the direction of separation.

Principle of HPLC columns

High-performance liquid chromatography – HPLC, is based on the classical chromatography cited the theory of gas chromatography. HPLC technically changes the mobile phase to the liquid transported under high pressure. Chromatographic columns are filled with a special method of small particle size filler so that the column efficiency is much higher than that of classical liquid chromatography.

Application of HPLC columns

HPLC is the most widely used chromatographic method and widely used in almost every field of quantitative and qualitative analysis. The high-performance liquid chromatography system is composed of mobile phase storage liquid bottle, infusion pump, sampler, chromatographic column, detector, and recorder. Its overall composition is similar to that of gas chromatography, but many adjustments have been made for its mobile phase as a liquid.

Feature of HPLC columns

The infusion pump of HPLC requires constant and stable infusion volume, and the injection system requires convenient and strict switching. As the viscosity of the liquid mobile phase is much higher than that of gas, in order to reduce the column pressure, HPLC columns are generally thicker and much shorter than gas chromatographic columns.

Product highlights and data evidence of HPLC

All the technique cores of HPLC from Hawach Scientific are focused on the characteristics of the chromatogram column.

Quality RecognitionThis is shown by the fact that terminal customers have published more than 500 papers in those journals at home and abroad. Famous oversea journals, there are Science, Journal of Chromatography A, Journal of Agricultural and Food Chemistry, and Journal of Pharmaceutical and Biomedical Analysis. etc., while domestic journals mainly involve the area of Fingerprinting Research with over 23 papers and 2 on Characteristic Fingerprinting.
Comparison With Similar Product of A Famous CompanyAccording to test results, the HPLC universal-C18 column of the Xchroma series has demonstrated excellent chromatographic column performance, notably in column efficiency and symmetry, which successively means that Hawach HPLC can substitute similar products from other international brands.
Application Advantage
  1. High column efficiency and large capacity of the peak
  2. Excellent batch stability
  3. High purity and good homogeneity of imported fillers
Multiple TypesSurprisingly, there are various types of only C18 columns such as the EChorma series (high purity porous spherical silica gel of type B) with a purity of 99.99%.