Anion HPLC Columns Characteristics and Operational Considerations

Anion-exchange high-performance liquid chromatography (HPLC) columns are used for separating and analyzing ions based on their charge characteristics. Here are some characteristics and operational considerations for anion HPLC columns:

Anion HPLC columns characteristics

(1)The Anion HPLC columns are high capacity, high efficiency, hydrophobic anion exchange column. Used to separate a wide range of valence anions, including polyphosphates, polyphosphates, and other multivalent complex reagents such as EDTA and NTA.
(2)Use polyphosphates, and chelating agents in complex sample matrices.
(3)Sulfide was determined using sodium hydroxide and wastewater samples for amperometric detection.
(4)Analysis of the use of hexavalent chromium columns after the reaction and environmental media absorption of visible light detection, including wastewater chromium, drinking water, and air.
(5)Separate inorganic and organic species of arsenic and urine samples in drinking water.
(6)High capacity, higher resolution.
(7)Longer retention times are required, but the resolution is higher.
(8)Promote rapid analysis of organic acids and anions in well-characterized samples.
(9)Particularly suitable for separating complex sample matrices or uncharacterized organic acids and anions in samples.
(10)Recommended for monovalent and divalent organic acids.

Anion HPLC columns operation precautions

(1) The column must be connected to the chromatograph after the column’s flow line is filled with the eluent.
(2) Connect the column to the direction of the flow path identified on the column.
(3) An appropriate amount of organic solvent may be added to the eluent for modification (less than 50% acetonitrile or methanol, etc.).
(4) The recommended temperature is 35C. It is recommended to use a column oven. The temperature has a certain correlation with the elution time of each ion. When the temperature changes, the elution time of the ions changes.
(5) After the pump is turned on, keep the flow rate below 0.5mLUmin, and then gradually increase the flow rate to the working flow.
(6) For samples containing organic matter and impurities, please pre-process the sample before injecting the sample.

Always refer to the specific guidelines provided by the column manufacturer and follow good laboratory practices for optimal performance and longevity of anion-exchange HPLC columns.